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This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. AAV6 relies more on sialic acid or sialic acid-containing glycoproteins than AAV1 for cell entry and/or subsequent steps of infection. Neuraminidases are enzymes that cleave via hydrolysis α(2-3)-, α(2-6)-, and α(2-8)-linked terminal […]
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They symbolize mid-factors in industrial reactions in multi-step processes, and in some cases can pace up chemical manufacturing by avoiding the need for earlier levels. Match up the necessities of your chemical technicians with listings on our marketplace, and stock up to ensure your entire reactions are lined. “Live attenuated” vaccines are made by reassorting […]
Terminal Sialic Acids are an Important Determinant of Pulmonary Endothelial Barrier Integrity
To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer, pH 7.5, in the presence 500 μm NADH. The structure was acquired from a crystal grown in the JCSG Plus screen (100 mm sodium citrate, […]